Zymoprep Yeast Plasmid Miniprep II, 50 Preps, Zymo Research, Mfr#: ZD2004

The Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, with no need for glass beads or phenol. The Zymoprep II recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.Simple: Quickly and easily rescue plasmid from yeast. Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids. High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.Storage Temperature: Room TemperatureStorage Conditions: Room TemperatureApplicable For: Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applicationsElution Volume: 10 μl Equipment: Microcentrifuge & Heat Block/Bath Processing Volume: 1.5 ml of CultureSample Source: Cell Culture (Colonies/Patches or Liquid Culture)Size Range: Up to 23 kb. Yield: Typically between 0.01-0.3 ng for most 2 μ based plasmids from 1.5 ml overnight culturesShipping Weight: 2 lbsShipping Dimensions: 7.00 x 5.00 x 4.50Research or further manufacturing use only, not for food or drug use.