X-GAL, 50 G, Rpi, Mfr#: B71800-50.0

Chromogenic substrate of Î2-Galactosidase used in combination with IPTG for detection of Î2-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by Î2-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show Î2-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of Î2-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.Water Content: 0.05% (K. Fisher)Protect from light and moistureCommon Name: 5-Bromo-4-chloro-3-indolyl-Î2-D-galactosideCAS Number: 7240-90-6Molecular Weight: 408.6Chemical Formula: C14H15BrCINO6Solubility: DMSO and DMFStorage Temperature: -20CStorage Conditions: FreezerAppearance: White Powder Identity: Conforms to structure Purity (HPLC): 98.0 %Solubility (2%, DMF): Clear and Colorless Solution Specific Rotation: -63.0 – -60.0 Water Content (K.F): 0.5 %BSE/TSE Free: BSE/TSE Free Country of Origin: Report Shipping Weight: 0.22 lbsShipping Dimensions: 2.50 x 4.75 x 2.50Research or further manufacturing use only, not for food or drug use.